Composite
Part:BBa_K2121007:Design
Designed by: Leslie Wang Group: iGEM16_Yale (2016-10-21)
Rhamnose Inducible Promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 215
Illegal SpeI site found at 105
Illegal PstI site found at 34 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 215
Illegal NheI site found at 4966
Illegal SpeI site found at 105
Illegal PstI site found at 34
Illegal NotI site found at 2840
Illegal NotI site found at 4373
Illegal NotI site found at 5663 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 215
Illegal BamHI site found at 354
Illegal BamHI site found at 1548 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 215
Illegal SpeI site found at 105
Illegal PstI site found at 34 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 215
Illegal SpeI site found at 105
Illegal PstI site found at 34
Illegal NgoMIV site found at 2285
Illegal NgoMIV site found at 2459
Illegal NgoMIV site found at 2509
Illegal NgoMIV site found at 2513
Illegal NgoMIV site found at 2540
Illegal NgoMIV site found at 3039
Illegal NgoMIV site found at 3397
Illegal AgeI site found at 87
Illegal AgeI site found at 5460 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1515
Illegal BsaI.rc site found at 787
Illegal SapI site found at 6054
Illegal SapI.rc site found at 5591
Design Notes
Utilize native activation mechanism in S. meliloti.
Construct design
Source
Mostafavi, Mina, et al. "Analysis of a taurine-dependent promoter in Sinorhizobium meliloti that offers tight modulation of gene expression." BMC microbiology 14.1 (2014): 1.