Composite

Part:BBa_K2121007:Design

Designed by: Leslie Wang   Group: iGEM16_Yale   (2016-10-21)


Rhamnose Inducible Promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 215
    Illegal SpeI site found at 105
    Illegal PstI site found at 34
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 215
    Illegal NheI site found at 4966
    Illegal SpeI site found at 105
    Illegal PstI site found at 34
    Illegal NotI site found at 2840
    Illegal NotI site found at 4373
    Illegal NotI site found at 5663
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 215
    Illegal BamHI site found at 354
    Illegal BamHI site found at 1548
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 215
    Illegal SpeI site found at 105
    Illegal PstI site found at 34
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 215
    Illegal SpeI site found at 105
    Illegal PstI site found at 34
    Illegal NgoMIV site found at 2285
    Illegal NgoMIV site found at 2459
    Illegal NgoMIV site found at 2509
    Illegal NgoMIV site found at 2513
    Illegal NgoMIV site found at 2540
    Illegal NgoMIV site found at 3039
    Illegal NgoMIV site found at 3397
    Illegal AgeI site found at 87
    Illegal AgeI site found at 5460
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1515
    Illegal BsaI.rc site found at 787
    Illegal SapI site found at 6054
    Illegal SapI.rc site found at 5591


Design Notes

Utilize native activation mechanism in S. meliloti.

Construct design Design_1.png

Design_2.png



Source

Mostafavi, Mina, et al. "Analysis of a taurine-dependent promoter in Sinorhizobium meliloti that offers tight modulation of gene expression." BMC microbiology 14.1 (2014): 1.


References